Google
Support independent publishing: buy this book on Lulu.

Wednesday, August 05, 2009

REGENERATIVE TEETH ALMOST PERFECT





Left:Picture taken from PNAS.


We have communicated previous upgrades notes around surprising reparative mechanisms of tails and members of salamanders and of dental regeneration of rabbits and certain dinosaurs (T. rex), always due to the constant emergency of blastemes (dental germs). Based on it, Japanese scientists have generated completely functional dental substitutions in lacking mature mice of molars teeths, by means of transplants of dental germs built by 3-D genetic engineering and inserted in alveolar bone with lost tooth. The inserted tooth erupted and developed in a new teeth with an almost perfect occlusion. Later analyses showed that the new tooth had correct dental structure, appropriate hardness and answer to noxious stimuli (mechanical stres and pain), etc.

The leader of the study Etsuko Ikeda (U/Tokyo), said that molar eruption was followed and identified by means of a green tint of fluorescein. The dental germs were built with epitelial and mesenchymal cells derived from mice’s embryos, mounted on a drop of collagen and cultivated 5-7 days. Then, the dental germs were implanted in the jaws of mice of 8 weeks to those that had been moved away (3 weeks before) one molar. The dental germs erupted 37-49 days later, being recognized in them: enamel, enamel forming cells, dentin, dentin cells, pulp, blood vessels, roots, cement,etc., besides generation of painful substances in front of appropriate stimuli. Although the molar teeth is not yet perfect, Japanese scientists said that they have yet human volunteers.



DIENTES A VOLUNTAD CASI PERFECTOS



Hemos comunicado en notas anteriores actualizaciones en torno a sorprendentes mecanismos reparativos de colas y miembros de salamandras y de regeneración dental de conejos y ciertos dinosaurios (T. rex), siempre debidos a la emergencia constante de blastemas (gérmenes), dentales. En base a ello, científicos japoneses han generado reemplazos dentales totalmente funcionales en ratones adultos carentes de huesos molares, mediante trasplantes de gérmenes dentales construidos mediante ingeniería genética 3-D, e insertados en el hueso alveolar, del diente perdido. El diente insertado erupcionó y desarrolló una oclusión dental, casi perfecta. Los análisis posteriores demostraron que el diente tenia una estructura dental correcta, dureza adecuada y respuesta a estimulos noxious (estres mecanico y dolor), etc.

El líder del estudio Etsuko Ikeda (U/Tokyo), dijo que la erupción del molar fué seguida e identificada mediante un tinte verde de fluoresceina. Los gérmenes dentales fueron construidos con células epiteliales y mesenquimales derivadas de embriones de ratones, montadas sobre una gota de colágeno y cultivadas 5-7 dias. Los gérmenes fueron implantados en las mandíbulas de ratones de 8 semanas a los que se les había retirado 3 semanas antes un molar. Los germenes dentales erupcionaron 37-49 días después, reconociéndose en ellos : esmalte dental, células formadoras de esmalte, dentina, células productoras de dentina, pulpa, vasos sanguíneos, raíces, cemento,etc., además de generación de sustancias dolorosas frente a estimulos apropiados. Aunque el molar aun no es perfecto, los cientificos japoneses refieren contar yá con voluntarios humanos.

Labels:

0 Comments:

Post a Comment

<< Home